*equally contributing     °corresponding author


The SZT2 Interactome Unravels New Functions of the KICSTOR Complex
Cattelani C, Lesiak D, Liebscher G, Singer II, Stasyk T, Wallnöfer MH, Heberle AM, Corti C, Hess MW, Pfaller K, Kwiatkowski M, Pramstaller PP, Hicks AA, Thedieck K, Müller T, Huber LA, Eca Guimaraes de Araujo M°
Cells. 2021; 10(10):2711.

The TSC Complex-mTORC1 Axis: From Lysosomes to Stress Granules and Back
Rehbein U, Prentzell MT, Cadena Sandoval M, Heberle AM, Henske EP, Opitz CA, Thedieck K°.
Front. Cell Dev. Biol. 9:751892. doi: 10.3389/fcell.2021.751892.

mTORC1 Crosstalk With Stress Granules in Aging and Age-Related Diseases
Cadena Sandoval M*, Heberle AM*, Rehbein U, Barile C, Ramos Pittol JM, Thedieck K°.
Front. Aging 2:761333. doi: 10.3389/fragi.2021.761333.

Combined Metabolic and Chemical (CoMetChem) Labeling Using Stable Isotopes
– a Strategy to Reveal Site-Specific Histone Acetylation and Deacetylation Rates by LC-MS

Alienke van Pijkeren*, Jörn Dietze*, Alejandro Sánchez Brotons*, Anna-Sophia Egger*, Tim Lijster, Andrei Barcaru, Madlen Hotze, Philipp Kobler, Frank J. Dekker, Peter Horvatovich, Barbro N. Melgert, Mathias Ziegler, Kathrin Thedieck°, Ines Heiland°, Rainer Bischoff°, and Marcel Kwiatkowski°.
Anal Chem. 2021 Sep 28;93(38):12872-12880. doi: 10.1021/acs.analchem.1c01359. Epub 2021 Sep 14.

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)
Klionsky DJ°, […], Heberle AM, […], Thedieck K, […]
For complete author list please click here.

Autophagy. 2021 Jan;17(1):1-382. doi: 10.1080/15548627.2020.1797280. Epub 2021 Feb 8.

G3BPs tether the TSC complex to lysosomes and suppress mTORC1 signaling
Prentzell MT*, Rehbein U*, Cadena Sandoval M*, De Meulemeester AS*, Baumeister R, Brohée L, Berdel B, Bockwoldt M, Carroll B, Chowdhury SR, von Deimling A, Demetriades C, Figlia G, Genomics England Research Consortium, Eca Guimaraes de Araujo M, Heberle AM, Heiland I, Holzwarth B, Huber LA, Jaworski J, Kedra M, Kern K, Kopach A, Korolchuk VI, van ‘t Land-Kuper I, Macias M, Nellist M, Palm W, Pusch S, Ramos Pittol JM, Reil M, Reintjes A, Reuter F, Sampson JR, Scheldeman C, Siekierska A, Stefan E, Teleman AA, Thomas LE, Torres-Quesada O, Trump S, West HD, de Witte P, Woltering S, Yordanov TE, Zmorzynska J, Opitz CA°, Thedieck K°
Cell. 2021 Feb 4;184(3):655-674.e27. doi: 10.1016/j.cell.2020.12.024

Finding new edges: systems approaches to MTOR signaling
Heberle AM*, Rehbein U*, Rodriguez Peiris M*, Thedieck K°
Biochem Soc Trans. 2021 Feb 5; BST20190730. doi: 10.1042/BST20190730


Podocytes maintain high basal levels of autophagy independent of mtor signaling
Bork T, Liang W, Yamahara K, Lee P, Tian Z, Liu S, Schell C, Thedieck K, Hartleben B, Patel K, Tharaux PL, Lenoir O, Huber TB.
Autophagy. 2020 Nov;16(11):1932-1948. doi: 10.1080/15548627.2019.1705007. Epub 2019 Dec 23.

IL4I1 Is a Metabolic Immune Checkpoint that Activates the AHR and Promotes Tumor Progression
Sadik A*, Somarribas Patterson LF*, Öztürk S*, Mohapatra SR*, Panitz V, Secker PF, Pfänder P, Loth S, Salem H, Prentzell MT, Berdel B, Iskar M, Faessler E, Reuter F, Kirst I, Kalter V, Foerster KI, Jäger E, Guevara CR, Sobeh M, Hielscher T, Poschet G, Reinhardt A, Hassel JC, Zapatka M, Hahn U, von Deimling A, Hopf C, Schlichting R, Escher BI, Burhenne J, Haefeli WE, Ishaque N, Böhme A, Schäuble S, Thedieck K, Trump S*, Seiffert M*, Opitz CA*°.
Cell. 2020 Sep 3;182(5):1252-1270.e34. doi: 10.1016/j.cell.2020.07.038

Breaking the Interface: Efficient Extraction of Magnetic Beads From Nanoliter Droplets for Automated Sequential Immunoassays
Lukas Metzler*, Ulrike Rehbein*, Jan-Niklas Schönberg, Thomas Brandstetter°, Kathrin Thedieck°, and Jürgen Rühe°.
Anal. Chem. 2020 Aug 4;161:90-99. doi: 10.1021/acs.analchem.0c00187


TGFβ-Signaling and FOXG1-Expression Are a Hallmark of Astrocyte Lineage Diversity in the Murine Ventral and Dorsal Forebrain
Weise SC, Villarreal A, Heidrich S, Dehghanian F, Schachtrup C, Nestel S, Schwarz J, Thedieck K, Vogel T.
Front Cell Neurosci. 2018 Nov 28;12:448. doi: 10.3389/fncel.2018.00448

Partially non-homogeneous dynamic Bayesian networks based on Bayesian models with partitioned design matrices
Shafiee Kamalabad M, Heberle AM, Thedieck K, Grzegorczyk M.
Bioinformatics. 2018 Nov 5. doi: 10.1093/bioinformatics/bty917

Upregulation of tryptophanyl-tRNA synthethase adapts human cancer cells to nutritional stress caused by tryptophan degradation
Adam I, Dewi DL, Mohapatra SR, Sadik A, Berdel B, Keil M, Sonner JK, Thedieck K, Rose AJ, Platten M, Heiland I, Trump S, Opitz CA.
Oncoimmunology. 2018 Sep 5. doi: 10.1080/2162402X.2018.1486353.

CGEF-1 regulates mTORC1 signaling during adult longevity and stress response in C. elegans.
Li Y, Finkbeiner S, Ganner A, Gerber J, Klein M, Grafe M, Kandzia J, Thien A, Thedieck K, Breves G, Jank T, Baumeister R, Walz G and Neumann-Haefelin E.
Oncotarget. 2018 Jan 6;9(11):9581-9595. doi: 10.18632/oncotarget.24039


Differential control of ageing and lifespan by isoforms and splice variants across the mTOR network.
Razquin Navas P, Thedieck K.
Essays Biochem. 2017 Jul 11;61(3):349-368. doi: 10.1042/EBC20160086.

Oncogenic β-catenin and PIK3CA instruct network states and cancer phenotypes in intestinal organoids.
Riemer P, Rydenfelt M, Marks M, van Eunen K, Thedieck K, Herrmann BG, Blüthgen N, Sers C, Morkel M.
J Cell Biol. 2017 Apr 25. doi: 10.1083/jcb.201610058.

PLK1 (polo like kinase 1) inhibits MTOR complex 1 and promotes autophagy.
Ruf S, Heberle AM, Langelaar-Makkinje M, Gelino S, Wilkinson D, Gerbeth C, Schwarz JJ, Holzwarth B, Warscheid B, Meisinger C, van Vugt MA, Baumeister R, Hansen M, Thedieck K.
Autophagy. 2017 Jan 19:0. doi: 10.1080/15548627.2016.1263781


Tumor-Intrinsic PD-L1 Signals Regulate Cell Growth, Pathogenesis, and Autophagy in Ovarian Cancer and Melanoma.  
Clark CA, Gupta HB, Sareddy G, Pandeswara S, Lao S, Yuan B, Drerup JM, Padron A, Conejo-Garcia J, Murthy K, Liu Y, Turk MJ, Thedieck K, Hurez V, Li R, Vadlamudi R, Curiel TJ.
Cancer Res. 2016 Dec 1;76(23):6964-6974.

A systems study reveals concurrent activation of AMPK and mTOR by amino acids.
Dalle Pezze P*, Ruf S*, Sonntag AG*, Langelaar-Makkinje M, Hall P, Heberle AM, Razquin Navas P, van Eunen K, Tölle RC, Schwarz JJ, Wiese H, Warscheid B, Deitersen J, Stork B, Fäßler E, Schäuble S, Hahn U, Horvatovich P, Shanley DP°, Thedieck K°.
Nat Commun. 2016 Nov 21;7:13254. doi: 10.1038/ncomms13254.


Functional proteomics identifies acinus L as a direct insulin- and amino acid-dependent mTORC1 substrate.
Schwarz JJ*, Wiese H*, Toelle RC, Zarei M, Dengjel J, Warscheid B°, Thedieck K°.
Mol Cell Proteomics. 2015 Apr 23. pii:mcp.M114.045807

TSC1 activates TGF-β – Smad2/3 signaling in growth arrest and epithelial to mesenchymal transition.
Thien, A*, Prentzel, MT*, Holzwarth, B, Klaesener, K, Kuper, I, Boehlke C, Sonntag A G, Ruf S, Maerz L, Nitschke R, Grellscheid SN, Reth M, Walz G, Baumeister R, Neumann-Haefelin E° , Thedieck K°
Developmental Cell. 2015 Mar 9; 32 (5):617–630. doi: 10.1016/j.devcel.2015.01.026. PubMed PMID: 25727005
→ View also the comment “TSC1 strikes out on its own” Sci. Signal., 24 March 2015 Vol. 8, Issue 369, p. ec66 doi: 10.1126/scisignal.aab1622

Nutrigenomics: Toward a Cross-Disciplinary Understanding of Nutrient-Driven Networks in Health and Disease.
Heiland, I,  Thedieck, K
Journal for Metabolic Research, Clinical Nutrition, and Dietetics. 2015; 40 (02), 88-92

Molecular mechanisms of mTOR regulation by stress.  
Heberle AM, Prentzell MT, van Eunen K, Bakker BM, Grellescheid SN, Thedieck K
Molecular and Cellular Oncology. 2015; 2 (2). doi: 10.4161/23723548.2014.970489


PI3K-p110-alpha-subtype signalling mediates survival, proliferation and neurogenesis of cortical progenitor cells via activation of mTORC2.
Wahane SD*, Hellbach N*, Prentzell MT*, Weise SC, Vezzali R, Kreutz C, Timmer J, Krieglstein K, Thedieck K°, Vogel T° .
J Neurochem. 2014 Jul;130(2):255-67. doi: 10.1111/jnc.12718. Epub 2014 May 3. PubMed PMID: 24645666.

T cell receptor-mediated activation is a potent inducer of macroautophagy in human CD8(+)CD28(+) T cells but not in CD8(+)CD28(-) T cells.
Arnold CR, Pritz T, Brunner S, Knabb C, Salvenmoser W, Holzwarth B, Thedieck K°, Grubeck-Loebenstein B °.
Exp Gerontol. 2014 Jun;54:75-83. doi: 10.1016/j.exger.2014.01.018. Epub 2014 Jan 24. PubMed PMID: 24468331.


Inhibition of mTORC1 by Astrin and Stress Granules Prevents Apoptosis in Cancer Cells.
Thedieck K°, Holzwarth B, Prentzell MT, Boehlke C, Kläsener K, Ruf S, Sonntag AG, Maerz L, Grellscheid SN, Kremmer E, Nitschke R, Kuehn EW, Jonker JW, Groen AK, Reth M, Hall MN, Baumeister R.
Cell. 2013 Aug 15;154(4):859-874. doi: 10.1016/j.cell.2013.07.031. PubMed PMID: 23953116.
→ View also the comment “Astrin Maintains Balanced mTORC1 Signaling under Cellular Stress” Cancer Discovery October 2013 3; doi: 10.1158/2159-8290.CD-RW2013-191


A dynamic network model of mTOR signaling reveals TSC-independent mTORC2 regulation.
Dalle Pezze P*, Sonntag AG*, Thien A, Prentzell MT, Gödel M, Fischer S, Neumann-Haefelin E, Huber TB, Baumeister R, Shanley DP°, Thedieck K°.
SCIENCE Signaling. 2012 Mar 27;5(217):ra25. doi: 10.1126/scisignal.2002469. PubMed PMID: 22457331.
→ View also the perspective “Deconvolution of mTORC2 “in Silico

Response to Comment on “A Dynamic Network Model of mTOR Signaling Reveals TSC-Independent mTORC2 Regulation”
Dalle Pezze, P*, Sonntag, AG*, Shanley, DP°, Thedieck, K°
SCIENCE Signaling. 2012 Jul 10;5(232):lc4.
*This is a related yet independent publication in which we respond with new data to several questions regarding the above study, raised by Prof. B. D. Manning, Harvard University, Boston, US.

A modelling-experimental approach reveals insulin receptor substrate (IRS)-dependent regulation of adenosine monosphosphate-dependent kinase (AMPK) by insulin
Sonntag AG,* Dalle Pezze P*, Shanley DP°, Thedieck K°.
FEBS J. 2012 Sep;279(18):3314-28. doi: 10.1111/j.1742-4658.2012.08582.x. PubMed PMID: 22452783.

2006 – 2011

Bile proteomic profiles differentiate cholangiocarcinoma from primary sclerosing cholangitis and choledocholithiasis.
Lankisch TO, Metzger J, Negm AA, Vosskuhl K, Schiffer E, Siwy J, Weismüller TJ, Schneider AS, Thedieck K, Baumeister R, Zürbig P, Weissinger EM, Manns MP, Mischak H, Wedemeyer J.
Hepatology. 2011 Mar;53(3):875-84. doi: 10.1002/hep.24103. Epub 2011 Jan 3. PubMed PMID: 21374660

Translational Control by Amino Acids and Energy.
Thedieck, K and Hall, MN
The Handbook of Cell Signaling, R. Bradshaw and E. Dennis (editors), 2nd edition, 2009.

Proteins induced by telomere dysfunction and DNA damage represent biomarkers of human aging and disease.
Jiang H, Schiffer E, Song Z, Wang J, Zürbig P, Thedieck K, Moes S, Bantel H, Saal N, Jantos J, Brecht M, Jenö P, Hall MN, Hager K, Manns MP, Hecker H, Ganser A, Döhner K, Bartke A, Meissner C, Mischak H, Ju Z, Rudolph KL.
Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11299-304. doi: 10.1073/pnas.0801457105. PubMed PMID:18695223.

PRAS40 and PRR5-like protein are new mTOR interactors that regulate apoptosis.
Thedieck K*, Polak P*, Kim ML, Molle KD, Cohen A, Jenö P, Arrieumerlou C, Hall MN.
PLoS One. 2007 Nov 21;2(11):e1217. PubMed PMID: 18030348; PubMed Central

The MprF protein is required for lysinylation of phospholipids in listerial membranes and confers resistance to cationic antimicrobial peptides (CAMPs) on Listeria monocytogenes.
Thedieck K*, Hain T*, Mohamed W, Tindall BJ, Nimtz M, Chakraborty T, Wehland J, Jänsch L.
Mol Microbiol. 2006 Dec;62(5):1325-39. PubMed PMID: 17042784


Method for modeling, optimizing, parameterizing, testing and/or validating a dynamic network or network perturbation.
Thedieck K, Sonntag AG, Dalle Pezze P,  Shanley DP.
Priority date June 1 2011, Publication n° WO2012163440.

Modulators of the interaction of astrin and raptor, and uses thereof in cancer therapy.
Thedieck K, Holzwarth B, Baumeister R.
Priority date Jan. 11 2013, Publication n° WO2014108532.